邢唷> {}z欹[ 鰢bjbj4螑螑%::8-(aw"ttt@'B'B'B'B'B'B'$*o-:f'ttf''   h@' @'  :&,&`2鑟歲+& ,''0-(&.-`-&-&0t0" tttf'f' ttt-(-ttttttttt:1 k:  1#1#8 #0!'%#2"'4 2 (Course Syllabus) #+1*'4 2 (Course Number) 3000757 3'+H'"4 (Course Credit) 2 credits 7H-'4 2 (Course Title) 2#A*---B#5 A%02#*1B#5#4*84L Protein Expression and Purification 0/ 2'4 2 (Faculty / Department) Faculty of Medicine 22#(6)2 (I/%2"/$9#I-) Semester (First / Second / Summer) 52#(6)2 (Academic Year) 2554 7H-9I*- (#2"'4 25H!59I*-+%2" #08 7H--22#"L9I#H'!*-8) (Instructor / Academic Staff) Prof. Alain Jacquet (alain.J@chula.ac.th) @7H-D#2"'4 2 (Condition) '4 25HI-@#5"!2H- (Prerequisite) - '4 211#H'! (Corequisite) - '4 2' (Concurrent) - *2 2-'4 2 ('4 211/'4 2@%7-) Status (Required / Elective) 7H-+%1*9# (Curriculum) '4 2#01 (Degree) M.Sc  Ph.D. 3' 1H'B!5H*-/*12+L (Hours / Week) 2 hrs. @7I-+2#2"'4 2 (Course Description) Protein expression in bacteria, in yeast, in insect cells, in mammalian cells. Techniques for protein purification including high-throughput protein production, chromatographic: ion-exchange, hydrophobic, size exclusion and affinity, protein isolation and characterization: ultrafiltration, precipitation, SDS-PAGE, Western blotting, ELISA, FACS, and overview on 2-D gel electrophoresis and mass spectrometry.#0!'%2#@#5"#2"'4 2 (Course Outline) '18#0*L1H'DA%0/+#7-'18#0*L@ 4$4##! (Learning Objectives / Behavioral Objectives) 1. -42" 4 A+%1- protein expression systems 2. -42" @4H2F5HC IC2# purification of proteins @7I-+2#2"'4 2H-*12+L (Learning Contents) Schedule :Time and venue : Lectures Thur 15.00pm -17.00 pm at room number 1012, Or Por Ror Building Fri. 10.00-12.00 am at room number 1012, Or Por Ror Building Online/ CAI are available for oversea students Lab session Tue.. 13.00-16.00 pm 14th Floor, Or Por Ror Building Fri. 13.00-16.00 pm 14th Floor, Or Por Ror Building Online/ CAI are available for oversea students All students who register to this course are required to attend the International Workshop on Protein Expression and Purification Strategies during 3-7 October 2011 at Chulalongkorn University, Bangkok, Thailand. Oversea students can choose to study online during the 1st 16th week. However, they must attend the International Workshop on Protein Expression and Purification Strategies during 3-7 October 2011 at Chulalongkorn University, Bangkok, Thailand. *** Oversea students can apply for Grant SupportWeek/dateTopics Instructors1 June 2, 2011 (2-hrs online is available)Introduction. Recombinant protein expression in bacteria Prof. Alain Jacquet 2 June 3, 2011 (2-hrs online is available)Recombinant protein expression in yeast Prof. Alain Jacquet 3 June 9, 2011 (2-hrs online is available)Recombinant protein expression in mammalian and insect cells Prof. Alain Jacquet 4 June 10, 2011 (2-hrs online is available)Protein detection and quantification (SDS-PAGE, Immunodetection, protein assays)Prof. Alain Jacquet 5 June 16, 2011. (2-hrs online is available)Protein purification Cell lysis, Precipitation, concentration, ultrafiltration, purification strategy, instrumentation, chromatographic techniques (part 1) ,Prof. Alain Jacquet 6 June 17, 2011 (2-hrs online is available)Protein purification Chromatographic techniques (part 2)Prof. Alain Jacquet 7 June 23, 2011 (2-hrs online is available)Protein purification Chromatographic techniques including fusion/tagged proteins (part 3)Prof. Alain Jacquet 8 June 24, 2011 3-hrs online is available)Lab session 1Prof. Alain Jacquet 9 June 28, 2011 3-hrs online is available) Lab session 2 10 July 5, 2011 (3-hrs online is available)Lab session 311-12 (4 hours)Homework 4hProf. Alain Jacquet 13 July 22,2011 (2 hours)Presentation16 July 29, 201Final ExamProf. Alain Jacquet 17. 3 - 7 October 2011. 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